Stimulation of Enzyme Reaction Rates by Crystalline Substrate Irradiation: Dependence on Identity of Irradiated Substrate

The study reported here concerns a phenomenon, discovered and extensively investigated by Sorin Comorosan, wherein enzyme initial reaction rates are enhanced as a consequence of incorporation of solutions derived from previously irradiated crystalline material into the reaction medium. Effective irradiation times conform to a sharply oscillatory pattern. In most reports, the irradiated crystalline material has been the substrate for the enzyme reaction to be studied, but there have been exceptions. The experiments presented here serve to confirm and extend this latter aspect of the phenomenon. It is found that the initial reaction rates for the lactic acid dehydrogenase (LDH) conversion of pyruvate to lactate can be stimulated by irradiation of crystalline deposits of sodium chloride, sodium bromide, potassium chloride and diatomaceous earth. Similarly, stimulation of the LDH conversion of lactate to pyruvate is demonstrated for irradiated sodium chloride. There appears to be no required chemical feature of the irradiated material other than crystalline state.